Cannabis HPLC Analyzer

Description

Included in the HPLC Package:

1. HPLC with temperature-controlled column compartment
2. 150mm C18 column with guard column holder and cartridge
3. Accessories for installation and maintenance
4. Intuitive HPLC software
5. Peak Auto Detection
6.  Proven, tested, and validated test methods
7. Calibration curves
8. Calibration log
9. Sample receiving log
10. Customer log
11. Maintenance log
12. Sample Preparation Protocols
13. Standard Operating Procedure (SOP)
14. Customizable Reporting System (Managed in MS Excel)
15. Easy-to-follow Instructions
16. Manual
17. Training
18. Technical Support

 

Starter Kit contains everything you need to get going right away. There are 29 items in the starter kit (major items listed below).

1. Commercial calibration standards
2. 1L HPLC buffer
3. 1L HPLC grade Methanol
4. 1L HPLC grade Acetonitrile
5. 1L HPLC grade Water
6. 3x100mL HPLC mobile phase bottle
7. 1x500mL HPLC mobile phase bottle
8. 100uL Hamilton microsyringe
9. 2.5mL Hamilton microsyringe
10. 2x5mL calibrated volumetric pipette
11. 15mL calibrated volumetric pipette
12. 20mL calibrated volumetric pipette
13. 100mL calibrated volumetric flask
14. Glassware
15. 100×1.8mL autosampler vials with PTFE/silicone cap
16. 100x16mL glass vial for extraction
17. Precision digital analytical scale (0.001g accuracy)
18. Nitrile gloves, labels

 

Lower Limit of Quantification (LLOQ)

The lower limit of quantification (LLOQ) is the lowest amount of a cannabinoid in a sample that can be quantitatively determined with suitable precision and accuracy using the corresponding method and dilution rates. All values below this threshold are reported as NR – None Reported.

Compound	Plant LLOQ (%,w/w)	High % Concentrate LLOQ (%,w/w)
Δ9-THC	0.03	0.04
Δ8-THC	0.03	0.06
Δ9-THCA	0.03	0.06
THCV	0.03	0.04
CBD	0.03	0.03
CBDA	0.03	0.03
CBDV	0.03	0.03
CBG	0.03	0.03
CBGA	0.03	0.03
CBN	0.03	0.03
CBC	0.03	0.05

Note: LLOQ values are dependent on sample dilution. These are values for standard methods but can be modified with dilution in case of need.

 

Reproducibility of results

Below are results of measuring a typical hemp sample, followed by an image of stacked 3 consecutive measurements. There are two observations from this measurement:

1. The retention times are consistent and repeatable, giving the confidence in the ability to correctly identify the correct cannabinoids.
2. The measured concentrations are very repeatable as indicated by height/area of each peak. The calculated relative standard deviation (RSD) in this case ranges from 1.19% to 1.70%, while for cannabinoids with very low concentrations manifested as very small peaks (in this sample CBN, d9-THC, and d9-THCA) the calculated RSD ranges from 4.62% to 5.63%. In case of need to further refine the latter 3 compounds and improve both accuracy and RSD, the sample would simply be less diluted resulting in higher peaks for these 3 compounds.